Cloned (Comment) | Organism |
---|---|
expression of the poly-His-tagged N-terminal fragment of the enzyme, i.e. BnDGAT1(1116)His6, in Escherichia coli strain BL21(DE3), BnDGAT1(1116)His6 fails to react with protein in microsomal vesicles following treatment with proteinase K, and thus might be localized to the cytosolic side of the ER | Brassica napus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | acyl-CoA substrate binding kinetics of the recombinant N-terminal fragment, overview | Brassica napus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Brassica napus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13278 | - |
x * 13278, recombinant poly-His-tagged N-terminal enzyme fragment comprising residues 1-116, sequence calculation | Brassica napus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-1,2-diacylglycerol + acyl-CoA | Brassica napus | - |
triacylglycerol + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant poly-His-tagged N-terminal fragment of the enzyme, i.e. BnDGAT1(1116)His6, from Escherichia coli strain BL21(DE3) | Brassica napus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-diacyl-sn-glycerol + erucoyl-CoA | - |
Brassica napus | 1,2-diacyl-3-erucoyl-sn-glycerol + CoA | - |
? | |
1,2-diacyl-sn-glycerol + oleoyl-CoA | - |
Brassica napus | 1,2-diacyl-3-oleoyl-sn-glycerol + CoA | - |
? | |
sn-1,2-diacylglycerol + acyl-CoA | - |
Brassica napus | triacylglycerol + CoA | - |
? | |
sn-1,2-diacylglycerol + erucoyl-CoA | i.e. a 22:1cisDELTA13-CoA | Brassica napus | triacylglycerol + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 13278, recombinant poly-His-tagged N-terminal enzyme fragment comprising residues 1-116, sequence calculation | Brassica napus |
More | analysis of self-associating properties of BnDGAT1(1116)His6 in an gel filtration approach, the recombinant N-terminal fragment associates to form tetramers | Brassica napus |
Synonyms | Comment | Organism |
---|---|---|
DGAT1 | - |
Brassica napus |
diacylglycerol acyltransferase-1 | - |
Brassica napus |